Nurses often anticipate future development and organise supplementary instruments and material in advance.
It is worth noting that most of these miRNAs have several validated target genes whose involvement regards different cancer pathways, p53 signalling, cell cycle control, and other pathways involved in cancer development and progression (Supplementary Table 2).
Consistent with previous findings, we identified the following lamin B1-dependent biological processes: cell cycle, chromosome segregation, response to oxidative stress and nervous system development (Table 1 and Supplementary Material, Tables S4 S6) (8, 16, 22, 23).
Gene ontology analysis revealed that many of the affected genes were involved in cell metabolism, signal transduction, cell cycling, immunity and defense, and development (Supplementary Figure S6).
The networks were specifically associated with cell signalling, cell adhesion, proliferation and growth, development and lipid metabolism (Supplementary Figure S1 and S2).
There is a 24-gene overlap between our 96-gene signature and that of Hoek et al., with 18 of the genes in common, including MITF, involved in melanin biosynthesis, pigmentation, development, and lineage specification (Supplementary Information S1), which reinforces the importance of these pathways in melanoma.
A relevant network that contains 9 of the 18 genes was obtained with an overrepresentation of the DNA Replication, Recombination and Repair, Cell Cycle, Connective Tissue Development and Function terms (Supplementary Figure S3a online).
This list of 89 high-confidence miR-10 targets in hNPCs contained several interesting transcripts, which are likely to influence brain development and neuronal patterning (supplementary material Table S5).
Gene Ontology (GO) analysis of DamID NKX2-5 targetshoweded that the most enriched biological processes were heart development and muscle contraction (Supplementary file 1), attesting to the specificity of DamID.
