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The objectives of this study were to sequence carrot ESTs using the Illumina platform and to characterize the carrot transcriptome to develop a molecular resource for marker development and gene identification.
Genomic approaches have recently provided 10,000 unigenes, which will help to develop genome-wide RNAi applications addressing fundamental questions in cereal weevil physiology, development and gene regulation.
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The energetic consequences of these mutations offer molecular targets and opportunities for new drug development and gene correction therapies.
The low efficiency of gene transfer is a recurrent problem in DNA vaccine development and gene therapy studies using non-viral vectors such as plasmid DNA (pDNA).
The availability of genetically altered cells is an essential prerequisite for many scientific and therapeutic applications including functional genomics, drug development, and gene therapy.
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Fig. 1B includes genes expressed during general T cell development and genes associated with myeloid lineages.
Many metabolic pathways have been implicated in its development, and genes associated with these pathways are altered.
The differently regulated ercc-1 transcripts were dominated by genes responding to stress, regulating growth and development and genes preferentially expressed in males (Supplementary Tables S1 and S2).
The major H4.1 subtype was expressed from five genes, with three expressed throughout development and genes at loci II and XII peaking at organogenesis.
Genes encoding homeobox transcription factors regulating early life development and genes involved in neural functions, particularly gated ion channels, were strongly modified by H3K27me3.
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