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To visualize embryos for microscopy, seeds containing embryos at different stages of development were collected from developing fruits and processed as previously described [ 36].
Developing fruits.
Expression profiling in leaves, petioles and developing fruits reveals distinct patterns, even within the same (sub class.
Overall, pre-dispersal losses were caused by wind and insect predation acting on flowers and developing fruits, whereas post-dispersal losses resulted from stratification during winter, and impact of microclimate and rodent predation upon seeds.
Significant increases in leaf number, area, weight per unit area, thickness and midrib angle, together with altered shape, have been recorded consistently for the leaves subtending the developing fruits of the columnar cultivars.
Approximately 20 developing fruits were gathered for this study.
Figure 1 The flowers and developing fruits of Cyrtosia javanica.
(B) The developing fruits of Cyrtosia javanica in the natural habitat.
Scale bar 1 cm Fig. 2 The flowers and developing fruits of G. nantoensis.
Scale bar 1 cm Fig. 3 The elongating stalks and developing fruits of various stages of G. nantoensis.
The major post-pollination developmental events that occurred in the developing fruits of two Gastrodia species are summarized in Table 1.
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