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In order to obtain the best reproducibility and separation, it is recommended to always use freshly activated chromarods and equilibrated solvent/ vapour phase in the developing chamber.
The mobile phase was poured in the developing chamber and was lined with filter paper and left as such for 30 min to make the chamber saturated with the vapors of mobile phase.
TLC developing chamber was lined with chromatography paper on all sides.
The developing chamber was saturated with the same solvent mixture from 10 min prior to development of the HPTLC plate.
The extract was air-dried on the TLC plate which was then placed in eluent in the developing chamber so the bottom of the plate is in the liquid.
The HPTLC plates were developed in toluene : AcOEt 7 : 3 (v/v) as mobile phase, in the automatic and reproducibly developing chamber ADC 2, saturated with the same mobile phase for 20 min at room temperature.
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Using the newly developed chamber, both PIXE and RBS analyses could be carried out at the same time and on the same point of the samples.
Despite the hours involved in developing the chamber and the struggles to finance it, Ms. Douglas said that when a member like Ms. Jones, the graphics company president, gets a contract with a major corporation, "it makes everything worthwhile, and I know I'm achieving the goals I've set".
While the remaining 15 become nurse cells, the oocyte relocalizes in a BicD-dependent manner to the posterior of the developing egg chamber.
To confirm our findings in another epithelial tissue, we examined the follicle cell epithelium that surrounds the developing egg chamber.
Strikingly, the Drosophila homolog of PCDH15, Cad99C, is required for microvilli stability in the developing egg chamber (Schlichting et al., 2006).
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