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One priority of any team involved in developing a transformation procedure is to identify the suitable target cell type.
Efficient regeneration protocols that could be applicable to a broad spectrum of different genotypes are a prerequisite for developing a transformation system for a plant species or genotype [ 1].
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This study developed a transformation based algorithm to solve a tri-level quadratic fractional programming problems using fuzzy goal programming approach.
Here, we report the first overexpression and detection of a heterologous sfCherry fluorescent protein in Nannochloropsis salina in order to develop a transformation toolbox for future genetic improvements.
In order to incorporate 3D fragmentation into subsequent conventional 2D analyses, we developed a transformation of this 3D fragmentation index into a series of 2D rasters based on index classes.
The aim of our study was not only to develop a transformation system for this important but recalcitrant species but to do so using a gene of interest and to study the behaviour of this transgene in a legume background.
These were used to develop a transformation of the healthcare system in Ontario, with creation of local health integrated networks, reorganization of the acute care system with increased capacity, development of infectious disease control.
We then developed a transformation method for L-forms and returned the murC and uppS genes, enabling the resumption of cell wall synthesis.
To overcome the challenges of mapping polymorphisms for fMAPK-independent adhesion, we developed a transformation protocol to select for plasmids carrying S288c genes that bypass the fMAPK pathway.
To increase the public acceptance of such transgenic plants we developed a transformation procedure that uses the chemical inducible Cre-lox system to obtain marker-free transgenic Jatropha.
We are currently testing these models to develop a transformation protocol that more faithfully reproduces the histology of human breast tumours.
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