Sentence examples for developing a restriction from inspiring English sources

Thus, we began developing a restriction enzyme guided genotyping-by-sequencing method termed Digital Genotyping (DG), when the 454 genome sequencing platform became available [ 21] and later transitioned this technology onto the Illumina GAIIx and HiSeq2000 to take advantage of their increased sequencing capacity [ 22].

We have developed a restriction fragment length polymorphism method that allows for determination of the 43-bp insdel (rs4795541) and A > G (rs25531) polymorphisms in the 5-HTTLPR promoter region within one assay.

Initially, we developed a restriction enzyme-based assay method in which T4- gt DNA was resistant to cleavage by MfeI after the glucosylation reaction.

Soon afterwards, we developed a restriction fragment length polymorphism PCR genotyping method for NAT2 (Hickman and Sim, 1991; Hickman et al., 1992).

Since restriction digests provide a fast and cost-effective method to rapidly screen PCR products, we developed a restriction endonuclease digestion assay of the amplification products to confirm that they correspond to the E6 and E7 viral genes.

We have developed a restriction enzyme-independent Tag-seq method for expression profiling (EXPRSS) and we present evidence that it performs better than existing restriction enzyme- based (SAGE and derivative) methods.

To confirm reduced methylation at position C2268 in nuclear 25S rRNA in nsun5 mutants, we developed a restriction enzyme digestion of PCR products using a dCAPs (derived cleaved amplified polymorphic sequences) primer derived from BS treated 25S rRNA.

Barchi et al. combined the recently developed a restriction site-associated DNA approach with Illumina DNA sequencing to rapidly discover a large number of SNP and SSR markers for eggplant.

We have developed a restriction enzyme targeted genome resequencing method for genetic analysis, termed Digital Genotyping (DG), to be applied to sorghum and other grass species with large repeat-rich genomes.

The aims of this study were to develop a restriction fragment length polymorphism (RFLP) assay for the single step rapid screening of individuals that carry first stretch 7-C at mitochondrial D310 region and to evaluate if any difference exists among healthy individuals and cancer patients in the Turkish population.

He said Test Central would try to work with the foundation to develop a formal restriction exempting nonprofit users from its patent.