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Compressive experiments were done to assess mechanical properties of developed scaffolds using Instron single column table top electromechanical tester (model 3345, Instron, Canton, MA).
We studied the in vitro biodegradation behavior (up to 4 weeks), mechanical properties and biocompatibility of the developed scaffolds.
While numerous studies have developed scaffolds for meniscus repair, the most appropriate autologous cell source remains to be determined.
Here, we characterize the microstructural and mechanical properties of these newly developed scaffolds with 50 and 75 wt.% mineral content.
Successfully developed scaffolds require mechanical and structural properties that match native vessels and optimal microenvironments that foster cell integration, adhesion and growth.
To this end, we developed scaffolds with covalently bound vascular endothelial growth factor (VEGF) and evaluated their mitogenic effect on endothelial cells in vitro.
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The results suggest the developed scaffold has the potential for use as a bone regenerative matrix.
Results from cell viability and cell proliferation with MG-63 cells using Alamar blue assay confirmed the cytocompatible nature of the developed scaffold.
Histological and immunohistochemical assessments further reveal that this multilayer scaffold has a significant capability of ectopic bone formation in vivo, enabling this newly developed scaffold to be suitable for wide applications in tissue engineering.
Compared to the scaffold without micro-ribs, the average compressive strength of newly developed scaffold was remarkably improved from 28.3 MPa to 45.6 MPa under the porosity of 50%.
This could suggests either that the developed scaffold material marginally influences cell proliferation in its direct vicinity or that the scaffold material exhibits a toxic effect on the ATDC5 cells, although no cell death was detected.
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