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Including previously developed primer sets, we report on the development of 10 polymorphic microsatellite loci for each species.
oryzicola, and B. glumae were detected in one PCR reaction that contained the newly developed primer set mix.
Among the specific markers developed, primer pair ZSBy8F5/R5 was specific for the By8 gene, which exists in Glu-B1b (Bx7+By8) and Glu-B1u (Bx7*+By8) alleles.
In silico and in vivo assays on four soil typologies were carried out to compare the newly developed primer sets with a selection of primers targeting the homologous gene, which were previously used to assess soil metazoan biodiversity.
To compensate for this limitation of dual-cured resin cements, some manufacturers have developed primer and adhesive solutions containing co-initiators to enhance the polymerization of the cement [10 12].
Some of the newly developed primer pairs appeared quite specific, others less so.
Similar(35)
Relative primer positions are compared between the newly developed primers described here and previously published ones.
We developed primers that are either universal or lineage-specific that successfully amplify matK across all fern families.
Smashbox has developed primers for the lid, under the eyes and the lashes, and is introducing one for the lips in 2013, according to the company.
In this study we developed primers for the amplification of GH28 coding genes from a database of 293 GH28 sequences from 40 fungal genomes.
We also present a case of identification of a crocodilian body part found in a local market using the newly developed primers.
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