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We developed Locus Explorer, a Shiny web application for R, to generate the locus plots shown in this manuscript.
To aid in the interpretation of data from this study, we developed Locus Explorer, a Shiny R application which allows the interactive graphical illustration of all the regions we have fine-mapped and which can be accessed at https://github.com/oncogenetics/LocusExplorer (manuscript in preparation).
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These two populations are widely separated and were selected to reveal the potential of the developed loci.
Furthermore, all loci were found to be polymorphic in all tested species (although for L. niger this information does not exist for the three newly developed loci).
Among the 31 isolates, eight to 12 well-supported clades were identified with the newly developed loci (Fig. 1, Appendices S1 S7).
With the newly developed loci, a sufficiently high number of highly variable microsatellite markers are now available for studies involving the characterization of the fine-scale population structure.
Establishment of the transferability of markers to other related species is therefore important while developing locus specific marker systems.
The developed root locus method can be used to study the steady-state behavior of any type of convergent biological system model based on mass action kinetics.
Linkage analysis revealed that 412 SSR loci (including 234 newly developed SSR loci) could be mapped to 17 linkage groups (LGs) covering 969.6 cM.
In this study, we constructed a microsatellite enriched library and developed microsatellite loci for future estimating the population genetic diversity based on microsatellite genotyping.
Three out of the 11 newly developed microsatellite loci (CpUZ001, CpUZ004, and CpUZ008) showed significant deviations from HWE (Table 2).
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