We characterized SNPs from orthologous A. mexicanum and A. t. tigrinum ESTs and extended this information to develop informative molecular markers for a related species, A. ordinarium.
In general, by using EST-derived primer pairs to amplify nuclear genome, the amplicons may consist of intron sequences that displayed higher variation to develop informative markers for variety identification (Shu et al. 2010).
Genomic-level questions can be addressed through developing informative transcriptome producing huge amounts of data [ 18, 19].
However, the frequency of valid SNPs for linkage analysis was low because of several difficulties in developing informative markers using the strategies described in this paper.
Among these methods, SAM can generate SSR markers generating multilocus SSR fingerprints, which requires only one pair of primers and has high efficiency in developing informative SSRs.
In view of the above, the present study was undertaken to develop informative TFGMS and TFFDMS markers from the TF genes and to evaluate their potential for large-scale genotyping applications in chickpea.
It would be thus interesting to develop informative InDel markers in silico at a genome-wide scale by comparing these publicly accessible genomic (gene) sequences of different chickpea accessions with sub-optimal use of resources.
The use of functional domains of TF genes for developing informative genic microsatellite markers in the case of two Solanaceous plant species, such as tomato and pepper, has been well documented.
For the development of support and care programs aimed at chronic patients, the strategy indicates that they must be limited to not only developing informative activities and training on caregiving for the dependent person, but also include interventions for the caregivers themselves, addressing their problems and preventing pathological conditions and the risk of giving up.
Recent developments in next-generation sequencing (NGS) technology have lowered the cost of sequencing per base, and enabled the genotyping by sequencing (GBS) approach for developing informative single-nucleotide polymorphism (SNP) markers in species with large, complex genomes (Elshire et al., 2011), including species without a reference genome sequence (Glaubitz et al., 2014).
