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The pipeline we have developed for assembly and analysis increases contig length, recovers unique transcripts, and assembles more base pairs than other methods through the use of a meta-assembly.
A generally applicable efficient method was developed for assembly of structurally defined sulfated oligosaccharides.
While most previous research was developed for assembly industries (where separation costs are easily calculated), this paper applies a new heuristic procedure to a basic problem in the meat industry.
In the first set of experiments we used a method developed for assembly of VP1 around DNA, except that DNA was omitted from the assembly reaction [41].
Among the various programs available, we validated publicly available program, Velvet (version 0.7.62; http://www.ebi.ac.uk/~zerbino/velvet/), which have been developed for assembly of short reads using de Bruijn graph algorithm.
To assemble the reads into transcripts, we applied the TGI Clustering Tools (TGICT), which is a set of utilities originally developed for assembly of data from cloned EST libraries.
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A similar tool, ABySS-Explorer has been developed for assemblies generated by ABySS [ 40].
A cellular manufacturing process was developed for fabrication and assembly of a full-scale biodiesel microreactor capable of producing biodiesel fuel at the rate of 2.47 L/min and at a capacity of over 1.2 million liters of fuel per year.
The assembly should be carefully evaluated because most of the assemblers were developed for genome assembly and are not designed to deal with the heterogeneity of metagenomic datasets.
Their procedures are usually executed at the end of the assembly process and are especially developed for repeat assembly by using paired-end information [ 12].
Programs developed for genome assembly and later adapted to transcript assembly (SOAPdenovo-trans, Oases [ 24]) seem to be less accurate than purpose-built transcriptome tools (such as Cufflinks and Trinity, personal communication).
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