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It was developed by Fuse Games for the Nintendo DS handheld game console, and released by Nintendo in North America and Australia in 2005, in Japan in 2006, and in Europe in 2007.
Hence, the system developed by Fuse at al. will make a good confirmatory assay, but may be too expensive for cell-based HTS.
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The tricistronic reporter construct was developed by fusing pieces from numerous different expression vectors.
Eleven fusion proteins were developed by fusing various truncated extracellular regions of FGFR1 with the Fc region of IgG1.
A specific blocker of IL-6/sIL-6R transactivation has been developed by fusing the extracellular domain of human gp130 to a human IgG1 antibody (sgp130Fc, Table 1).
Toward this goal, single-chain FokI TALEN variants have been developed by fusing two FokI monomers with a polypeptide linker that is then fused to the TALE domain (Sun and Zhao 2014).
The Cas9/gRNA system is developed by fusing 2 small RNA (CRISPR RNA and transactivating crRNA) to form a gRNA, which guides the Cas9 protein to cleave specific DNA [ 23, 26].
Based on the functional dissociation of transcription factors into a DNA-binding domain and a transcription regulatory domain, genetic scissors have been developed by fusing DNA-binding domains to the catalytic domain of endonucleases.
More recently, one decoy receptor fusion protein, FP-1039, which was developed by fusing the whole extracellular region of FGFR1 with IgG1 Fc, has been shown to be effective in preclinical as well as clinical trials (Harding et al, 2013).
Furthermore, a mouse model that enables temporal control of Myc activation after tamoxifen treatment was developed by fusing the Myc gene to the hormone-binding domain of the estrogen receptor (ER) (Eilers et al., 1989), which was used to investigate the effect of timed Myc activation in distinct tissues.
In the past decade, two major formulations of subunit vaccines consisting of F1 and LcrV antigens were developed by such methods as mixing both purified antigens (F1+V) or fusing them together (F1−V).
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