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MβCD-treated (n = 27) and control (n = 12) female animals were allowed to develop to stage 6 so that we could analyze the behavior of ORN axons as they extended through the SZ and formed glomeruli.
The Tyr-TALEN-mRNA-injected embryos and mRNA-injected and host-transferred oocytes were allowed to develop to stage 39 40 tadpoles.
Gemmules were allowed to develop to stage 5 before they were treated with either dsRNA or fed bacteria to ensure that feeding structures had fully developed.
Each set of injected embryos was incubated on cover glasses at different temperatures (18°C, room temperature, or 25°C) so that they would develop to stage 10 11 at approximately the same time.
Deltats embryos were shifted for 3 hours to the restrictive temperature to induce supernumerary IPC NBs and then shifted back to permissive temperature and allowed to develop to stage 16.
Animals were allowed to develop to stage 14 (when glomerular architecture has been well established and ORN axon ingrowth is complete; n = 3) or to stage18 (mature; n = 4).
Similar(54)
The Ballantyne staging system, which was originally developed to stage head and neck melanomas, is generally used to stage anorectal and vaginal melanomas (Table 2) [24 25, 25].
The embryos were harvested for immunofluorescence or real-time analysis at 3 days after the infusion, at which time they had developed to stage 25 26 and had begun to develop organs such as heart, brain and liver.
Compared with other techniques that have been developed to stage axillary lymphatic node metastases, including dynamic contrast-enhanced MRI and diffusion-weighted imaging techniques, Gd-MRL is more accurate [ 30- 32].
Numerous noninvasive panels of tests have been developed to stage liver disease, including a combination of clinical and routine laboratory parameters, as well as specialized tests involving direct markers of fibrosis and elastography [ 9- 20].
Embryos were allowed to develop to stage-29/30 stage-29/30xtent of cardiac differentiation in embryos whenvisualized as the symmextentl flat plates expressing TIc and/of Nkx2.10 (not shown), either side of the ventral midline.
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