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After a few weeks festering in a cardboard box behind my desk, the fruits we originally took to St Anne's have started to develop shoots.
As is evident in these studies of the carbon budget of deciduous woody plants, carbon reserves are required in spring to develop shoots from dormant buds (Loescher et al. 1990; Bennett et al. 2005; Holzapfel et al. 2010).
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After eight international appearances, Agdestein was forced to retire from football at the age of 23 because of a knee injury, and without a physical outlet, he was no longer able to concentrate for long periods of time, and would often hyperventilate or develop shooting pains in his arms during chess matches.
They are discernible in the leaves of autumn foliage and in developing shoots.
Shoot development was enhanced by 10 μM BAP and 10 μM AgNO3 leading to 100% well developed shoots.
After 1 2 weeks of culture, the somatic embryos developed shoots and subsequent turned into 1-cm-height plantlets for another 2 weeks (Fig. 2c).
Vascular connections were also detected between developing shoots and callus tissue.
Guttation of developing shoots clearly shows that E. giganteum is able to generate substantial root pressure, even when its water source is moderately saline, and can selectively channel this pressure to developing shoots (Fig. 2B).
This might suggest that the actively growing meristem cells can influence surrounding cells inducing them to differentiate into developing shoots.
-Regeneration percentage (R): number of cultures that differentiated into completely developed shoots × 100/total number of cultured explants.
Developing shoots were subsequently transferred to a root initiation medium CR5 [ 36] before finally being planted into potting mix in the greenhouse.
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