Sentence examples for develop restriction from inspiring English sources

To develop restriction fragment length polymorphism (RFLP) assays from these snip-SNPs, we designed PCR primers with a standard protocol and performed in silico digests of the resulting amplicons to infer the banding patterns for each strain.

The objective of this paper is to develop restrictions on the constitutive equations in the linear Cosserat theory of rigid heat conducting shells which ensure that the Cosserat equations produce exact steady-state solutions for Fourier conduction with an arbitrary constant temperature gradient for all shell geometries including variable thickness.

These aspects should be carefully considered when countries develop restrictions and limitations of foreign immigration.

The principle of developing restriction operator is based on the evaluation of the residuals on the coarse grid level with the use of residuals on the fine grid level.

We developed restriction fragment length polymorphism (RFLP) assays to detect sequence differences discriminating the intact APBA2 locus from the partial duplications.

We have combined the recently developed restriction-site associated DNA (RAD) approach with the Illumina DNA sequencing platform to effect the rapid and mass discovery of SNP markers for C. cardunculus.

As one of several methods that are based on NGS platforms to develop markers, restriction site-associated DNA sequencing (RAD-Seq) obtains sequences of restriction enzyme digestion tags using Illumina sequencing.

The shorter distance of fixation from the original insertion may decrease the effect of weakening, but did not develop much restriction.

The aims of this study were to develop a restriction fragment length polymorphism (RFLP) assay for the single step rapid screening of individuals that carry first stretch 7-C at mitochondrial D310 region and to evaluate if any difference exists among healthy individuals and cancer patients in the Turkish population.

Genotyping through the sequencing of reduced representation genomic libraries developed through restriction digestion of genomic DNA (restriction-site associated DNA; RAD) was initially proposed by Miller (2007) [ 31] and adapted to incorporate barcoding for multiplexing with Illumina sequencing technology by Baird et al. (2008) [ 32].

Since restriction digests provide a fast and cost-effective method to rapidly screen PCR products, we developed a restriction endonuclease digestion assay of the amplification products to confirm that they correspond to the E6 and E7 viral genes.