Based on the CA-GFP platform, we developed reporters that can discriminate the activities of caspase-6 and -7, two highly related proteases.
In order to address issues of assaying ease we are developing reporters of kinase activity that exploit the chelation-enhanced fluorescence of the sulfonamido-oxine (Sox) family of chromophores.
Its bioluminescence signal intensity greatly surpasses previously developed reporters for Chlamydomonas nuclear gene expression and reaches values high enough for utilizing the reporter as a tool to monitor responses to environmental stresses in vivo and to conduct high-throughput screenings for signaling mutants in Chlamydomonas.
Using a newly developed reporter system, it was found that expression of arcA and etrA was not influenced by growth conditions but transcription of crp was induced by removal of oxygen.
We have developed reporter gene assays for human androgen receptor (hAR) and human estrogen receptor-alpha (hER alpha) using Chinese hamster ovary cells.
We previously developed reporter-peptide nucleic acid (PNA -peptides for sequence-sPNA -peptidesimaging and fororescence imaging of particular mRNAs in cells and tumoradioimaging
To find such regulators whose mutations enhance transgene expression in Caenorhabditis elegans, we took advantage of a newly developed reporter transgene, lin-11pAΔ:: venus.
The G-Luc reporter outperforms previously developed reporter genes for Chlamydomonas, improves the monitoring of gene expression and, most importantly, represents the first nuclear reporter gene that is sufficiently sensitive to facilitate in vivo imaging in Chlamydomonas.
Developing reporter systems such as luciferase, beta-galactosidase (LacZ), or fluorescent proteins (FPs) are useful for two reasons: (i) they provide experimental information on putative regulatory DNA sequences, and (ii) they provide information on tissue- and stage specificity of gene expression.
A more recent developed reporter strain for detecting long-chain AHLs ranging from 3-oxo-C6-HSL to C14-HSL is C. violaceum VIR24, an in-frame deletion mutant of the cvil gene encoding AHL synthase in C. violaceum ATCC12472 [ 97].
