On an afternoon two years later, a sixteen-frames-a-second movie of the investiture of the Prince of Wales was developed in a darkroom on a British express train and taken to London, where it was shown the same night.
He shoots on actual film, which he develops in a darkroom, and compared with the cellphone grabs and telephoto peeping Tom shots that dominate the Web, many of his pictures do have an artisan quality that suggests at least some tentative residence in the realms of fine art.
This was followed by incubation with peroxidase-labelled goat anti-rabbit secondary antibody (Cell Signaling Technology; 1 10,000) for 60 min at room temperature, before the membrane was developed in a darkroom using ECL reagent (Millipore), scanned and quantified by densitometry (ImageJ).
This was followed by incubation with Alexa Fluor® 594 goat anti-mouse (Molecular Probes; 1 10,000) and horse anti-mouse (Cell Signaling Technology; 1 10,000) secondary antibodies, respectively, for 60 min at room temperature, before membranes were developed in a darkroom using ECL reagent (Millipore), scanned and quantified by densitometry (ImageJ).
Real photo cards were usually produced in very small quantities, because they had to be developed in a darkroom, one by one.
Each print, developed in a makeshift darkroom, required 15 chemical steps, and along the way he added retouching pigments to produce the illusions of neat clouds in the skies.
Virtues develop in the darkroom that nobody on set knew were there.
And Father develops photos in a darkroom.
