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As larvae metamorphose, they develop digits from their limb bud protrusions.
Their analyses indicated that digits have been regained in some species of this clade and, accordingly, that the ability to develop digits may be preserved for an extended period after their loss.
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Although Hoxd8 and Hoxd9 mainly contribute to proximal limb development and are only mildly expressed in the developing digits, we found that Hoxd8 and Hoxd9 were highly expressed in bat wing digits II-V relative to the thumb and hindlimb digits [ 39].
Using next generation sequencing technology, we performed digital gene expression tag profiling (DGE-tag profiling) of developing digits in a pooled sample of two Myotis ricketti and validated our sequencing results using real-time quantitative PCR (RT-qPCR) of gene expression in the developing digits of two Hipposideros armiger.
Later in development, strong expression of mROR2 is seen within the perichondrium of the developing digits, while mROR1 expression is seen in the necrotic and interdigital zones (Al-Shawi et al., 2001; Matsuda et al., 2001).
Among the key players, Hoxd genes are coordinately regulated in developing digits, where they help organize growth and patterns.
From HH st32 weak expression is observed around the distal tips of the developing digits (Figure 2O).
During this second phase, Hoxd13 is expressed in all of the developing digits whereas Hoxd12 and Hoxd11 are expressed in all but the anteriormost digit.
We cloned a fragment of exon 1 of HoxD-11 of the crocodilian Alligator mississippiensis and observed its transcription in developing digits.
The posterior HoxD genes (i.e. HoxD-10, HoxD-11, HoxD-12, and HoxD-13) are well known for their expression and function in developing digits [9], [10].
To validate gene expression in the developing digits of two individuals of Hipposideros armiger, DGE-tag profiling of developing digits in a pooled sample of two Myotis ricketti was analyzed [ 12].
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