Sentence examples for develop a restriction from inspiring English sources

The aims of this study were to develop a restriction fragment length polymorphism (RFLP) assay for the single step rapid screening of individuals that carry first stretch 7-C at mitochondrial D310 region and to evaluate if any difference exists among healthy individuals and cancer patients in the Turkish population.

We have developed a restriction fragment length polymorphism method that allows for determination of the 43-bp insdel (rs4795541) and A > G (rs25531) polymorphisms in the 5-HTTLPR promoter region within one assay.

Initially, we developed a restriction enzyme-based assay method in which T4- gt DNA was resistant to cleavage by MfeI after the glucosylation reaction.

Soon afterwards, we developed a restriction fragment length polymorphism PCR genotyping method for NAT2 (Hickman and Sim, 1991; Hickman et al., 1992).

Since restriction digests provide a fast and cost-effective method to rapidly screen PCR products, we developed a restriction endonuclease digestion assay of the amplification products to confirm that they correspond to the E6 and E7 viral genes.

We have developed a restriction enzyme-independent Tag-seq method for expression profiling (EXPRSS) and we present evidence that it performs better than existing restriction enzyme- based (SAGE and derivative) methods.

To confirm reduced methylation at position C2268 in nuclear 25S rRNA in nsun5 mutants, we developed a restriction enzyme digestion of PCR products using a dCAPs (derived cleaved amplified polymorphic sequences) primer derived from BS treated 25S rRNA.

Barchi et al. combined the recently developed a restriction site-associated DNA approach with Illumina DNA sequencing to rapidly discover a large number of SNP and SSR markers for eggplant.

We have developed a restriction enzyme targeted genome resequencing method for genetic analysis, termed Digital Genotyping (DG), to be applied to sorghum and other grass species with large repeat-rich genomes.

Thus, we began developing a restriction enzyme guided genotyping-by-sequencing method termed Digital Genotyping (DG), when the 454 genome sequencing platform became available [ 21] and later transitioned this technology onto the Illumina GAIIx and HiSeq2000 to take advantage of their increased sequencing capacity [ 22].

He said Test Central would try to work with the foundation to develop a formal restriction exempting nonprofit users from its patent.