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We have developed straightforward protocols for the establishment of human embryonic stem (hES) cell-derived mesenchymal progenitor (hES-MP) cell lines.
We developed an efficient procedure for the establishment and evaluation of stable cell lines for anti-FMDV research based on RNAi technology, which can be a candidate method for anti-FMDV research.
The optimized protocol for the KG-1 cell line is available at http://www.lonzabio.com.
Quality assurance was developed through the adoption of a protocol for the management of malnutrition, the establishment of training programmes for its use, and the design of a monitoring system.
PP and GM provided protocols for tissue acquisition and storage for the establishment of cell lines and tissue cultures; OAR and MH provided protocols in Appendices 1 and 2. All authors read and approved the final manuscript.
Also, in 2005 we successfully developed a differentiation protocol for cardiovascular cells including cardiac muscle and angiogenic cells.
Written informed consent had been signed for the establishment of cell lines as well as subsequent studies, and this project was approved by the Ethics Committee at Chinese Academy of Medical Sciences and Peking Union Medical College.
The authors wish to thank Dr. Renald Gilbert for providing protocols for establishment of stable cell line; Dr. Gary Levy and Andre Siegel for providing support in recombinant protein purification.
We have developed a protocol to expand human GDTc in vitro and confirmed their selective cytotoxicity to Ph+ leukemia cell lines.
The Marble Institute of America plans to develop a testing protocol for granite.
We failed to develop a potential permanent cell line; however, the results obtained allow us to assert that some tumor-like formations can be useful objects for establishment of permanent cell lines.
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