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To further develop a marker that can be used to discriminate Pi2 from Piz-t or from other non-Pi2 alleles, we focused on the polymorphic sites within the coding regions of Pi2 and Piz-t.
Based on the multiple sequence alignment of 26 sequences of the promoter region of Pi9 and its alleles, primers 9-Pro-F/9-Pro-R were designed to develop a marker targeting the InDel polymorphisms among the Pi2/Pizt, Pi9, and non-Pi2/Piz-t/Pi9 alleles.
To our best knowledge, this is the first investigation to develop a marker panel for improved recurrence prediction in HCC patients treated with LT.
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In this research, rice (Oryza sativa L). was used as a model to develop a marker-genotyping system to meet the technical challenges, and the seed dormancy QTL SD 1-2, SD 7-1, and SD 12, which were isolated as single Mendelian factors into the same genetic background in the previous research, were selected to demonstrate the efficacy of the new genetic approach.
Unlike MacDonald, Gusella thinks that a biomarker would not be particularly meaningful; rather, he would like to see researchers direct their efforts toward developing a marker that could indicate a reversible disease state.
In conclusion, we have developed an original approach coupling bioinformatic and immuno-epidemiological tools, which succeeded in identifying a candidate for developing a marker of exposure to An. gambiae bites.
When developing a marker set for parentage assignment, it is important to be able to predict the assignment efficiency from a priori data.
Radiation hybrid mapping is an important tool for mapping/cloning genes in recombination poor regions [ 14] and developing a marker scaffold for whole genome assembly [ 8, 25] of important crops like wheat with complex and highly repetitive genomes.
The application of our results in pear rootstock breeding may assist in developing a marker for MAS selection for breeding a pear rootstock that confers both vigour control and precocity to the grafted scion cultivar.
To accomplish this, we developed a marker panel densely covering the 1.4 Mb DYX2 locus and assessed association with reading, language, and IQ measures in subjects from the Avon Longitudinal Study of Parents and Children.
We developed a marker-free transformation pipeline to select potato plants functionally expressing a stack of late blight R genes.
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CEO of Professional Science Editing for Scientists @ prosciediting.com