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Meanwhile, Tiny Tower developer NimbleBit criticised (but stopped short of suing) Zynga for its Dream Heights game, suggesting that rebuffing the latter's acquisition feelers had led Zynga to develop a clone instead.
They used their spare time late at night to develop a clone of the first level of Super Mario Bros. 3, using the hero in Softdisk's Dangerous Dave, calling the prototype Dangerous Dave in Copyright Infringement.
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Our aim was to develop a cloning strategy that allows to generate any gene of interest (GOI) as an N- or C-terminal EYFP/ECFP-fusion without further modifications of the vectors (Fig. S1).
The Tokyo-based company responsible for this experiment is called KLab, and it has developed a clone of the Kindle Developer's Kit (KDK) and Kindle Emulator.
In 1983 ITRI developed a clone of the IBM PC.
Just like the Storm before it, RIM is developing a clone of an Apple device.
In this study, we developed a clone of human neuroblastoma cell line SH-SY5Y overexpressing APP751wt.
To circumvent the above-mentioned problems, we developed a cloning method termed FastCloning.
To circumvent the laborious and cost-intensive duplication of all processes during clone generation and quality control, we developed a cloning strategy that allows for the simultaneous generation of two variants of Gateway entry clones, one containing a particular ORF with and the other without the stop codon.
Prior to the construction of the full-length enriched library we developed a cloning vector (pENTR- SfiI) allowing for both effective directional cloning by taking advantage of the nonsymmetrical cleavage of the SfiI restriction enzyme and for the ease of subcloning provided by the Gateway System.
We then decided to develop a novel cloning method independent of restriction site and ligase.
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