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Overall, the results show that for rolling deformation external strain accommodation, rather than self-strain accommodation, is the dominant factor in determining the variant types of the double twins formed during warm rolling.
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In order to determine the variant contribution, we propose the use of the Analytical Hierarchical Process(AHP) method (Saaty 1987).
To determine the variant density along the genome, the mitochondrial genome of the parental strains was binned into adjacent 1-kb windows.
To determine the variant quality score threshold, we measured genotype concordance between individual-matched WGS from lymphoblast and fibroblast samples from seven individuals, which are expected to have minimal discrepancies.
Based on the process costs, we can derive the periodic profit margin M t, as shown in Eq. (7) (ESM of Appendix A.2). We therefore determine the variant-specific periodic process costs by inserting the cumulated variant-specific demand into the linearly approximated experience curve from Eq. (6), including the master process as a particular variant.
Investigation at the genomic scale is required to determine the variants affecting the regulation of the hormones studied.
Determining the causative variant was then easily achieved through standard segregation approaches.
These results demonstrate that the amplicon sizing assays are suitable for determining the dominant variant in LH mixtures up to a ratio of 95%.
Further fine mapping studies will be required to determine the causal variant or variants.
Evaluating such signals and determining the real causal variant will, however, be a difficult task.
This study also confirmed the crucial role of the trans-associated mutation in determining the pathogenicity of variant R229Q.
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