We examined the course of apoptosis during the rewarming period following the cold-shock on ice by determining the staining with labeled AnV and propidium iodide (PI).
Using this method of assessment, we evaluated the expression of MTUS1 by determining the staining index, which scores as 0, 1, 2, 3, 4, 6, and 9.
Using this method of estimation, we evaluated expression of LNβ3 in the tumor and adjacent normal pancreatic ductal tissue by determining the staining index with scores of 0, 1, 2, 3, 4, 6, or 9.
Using this method of assessment, we evaluated SAM68 expression in MIBC tissues by determining the staining index, with scores of 0, 1, 2, 3, 4, 6, 8, 9, and 12. SAM68 threshold values were chosen on the basis of a measure of heterogeneity with the log-rank test statistical analysis with respect to recurrence-free survival.
Using this method of assessment, we evaluated the expression of MACC1 in benign breast epithelium and malignant lesions by determining the staining index, resulting in scores as 0, 1, 2, 3, 4, 6 or 9. Cutoff values for MACC1 were chosen on the basis of a measurement of heterogeneity with the log-rank test statistical analysis with respect to overall survival.
The positive slides were further evaluated to determine the staining intensity.
The sum of the staining intensity and positive cell scores was used to determine the staining index for each section.
In brief, we first determined the staining grade I: 0, no staining; 1, pale yellow; 2, brown yellow; 3, brownish.
We therefore determined the staining pattern of p65/RELA in the cytoplasmic or nuclear compartment of airway epithelial cells.
The slides were screened at a low power for pattern and distribution of staining; a higher magnification (×40) was used to determine the staining intensity.
Example sections and sections used to determine the staining intensity of zebrin II were photographed using a Leica DMRB microscope equipped with Leica DC300 camera.
