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In 1977 two different methods were invented for determining the nucleotide sequence of DNA: one by American molecular biologists Allan Maxam and Walter Gilbert and the other by English biochemist Fred Sanger.
By determining the nucleotide sequence of the amplicon, a molecular typing method was developed.
Objective: To identify the major dermatophytes, Trichophyton rubrum, T. mentagrophytes, T. violaceum, M. gypseum, M. canis and E. floccosum, by PCR amplifications at the species level, without determining the nucleotide sequence.
Alleles were identified by determining the nucleotide sequence of the amplified fragment.
Determining the nucleotide diversity of these two genes in wild barley would allow verification of this hypothesis.
MLST was performed by determining the nucleotide sequences of 7 housekeeping genes (abcZ, adk, aroE, fumC, gdh, pdhC, pgm) as previously described (8 ).
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From our study and the identity level of plant miRNA orthologs, the number of 17 nucleotides of the miRNA chosen for primer design was workable and reasonable in determining the nucleotides flanking the last nucleotide in the predicted miRNA for this design would allow at most three and four mismatched nucleotides.
DNA sequencing, technique used to determine the nucleotide sequence of DNA (deoxyribonucleic acid).
We cloned these specific bands and determined the nucleotide sequences.
We determined the nucleotide sequences of the ITS1 ribosomal DNAs and designed species-specific primers for each species.
To determine the nucleotide sequence of the 16S rRNA gene, genomic DNA was extracted from 5-day cultured cells using the QIAGEN® Genomic DNA Kit.
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