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Experimentally determining the expression of miRs, we found an over-representation of over under -expressed miRs with variover under -expressedrget sequences.
Therefore, the potential of hAMSCs to undergo malignant transformation into TAFs when exposed to GBM CM was assessed by determining the expression of vimentin and alpha sm-actin, both proteins that are highly expressed in TAFs.
These results demonstrate an easy and simple method for determining the expression of chimeric fusion genes with no preamplification.
The role of each hormonal substance in determining the expression of the tail vibration behavior was discussed based on the results.
Here we examine the role of inflammatory mediators in Epac1-induced cellular proliferation by determining the expression of the pro-inflammatory markers p-cPLA2, COX-2, and PGE2 in prostate cancer cells treated with 8-CPT-2Me-cAMP.
Then, we evaluated EMT in the fibrotic kidneys of the RBP-J cKO and control mice by determining the expression of the EMT-related markers using qRT-PCR and Western blotting.
We were interested in determining the expression of these subunits and the activation of these proteins as follicles grow.
A similar threshold for considering a tumour Snail1 positive has been used by other authors [14] when determining the expression of this factor in ovarian tumours.
These authors concluded that cancer associated signals are more important than germline genetic variability in determining the expression of PAI1 in breast cancer patients.
Determining the expression of UTP14C by RT-PCR is complicated by the fact that it is located within an actively transcribed host gene, GT8.
For determining the expression of SOD in B. neotomae and in the complemented strain, SDS-PAGE and Western blot analyses were performed as described previously [18], [19].
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