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In addition to the Melt® procedure, migrating the PCR products on an agarose gel can also help in determining specificity of the amplification procedure.
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Western blotting and immunohistochemistry were used to determine specificity of the VANGL1 antibody (Fig. S5).
To determine specificity of the PCR, a sample of human DNA from the LNCaP prostate cancer cell line (American Type Culture Collection, code CRL-1740) wamplifiedied with the XMRV and MLV primer sets.
Following amplification, melting temperatures of PCR products were analyzed to determine specificity of the PCR product.
To determine specificity of the primers, 51 isolates were also subjected to the LAMP assay.
These experiments were just additional controls to the standard IgG controls to determine specificity of the interaction between uPAR and HSP70 and MRJ.
For E. coli isoacceptors with overlapping codon specificity the parameters for the tRNA fraction that pairs to each codon were calculated according to the experimentally determined specificities of the ternary complexes [23], [24], [27], [52].
Finally, we determined specificity and sensitivity of the HER-2 domains' targeting by flow cytometry (Fig. 2).
We began by determining the specificity of the canonical Notch and Wnt pathways in activating Müller cells with dormant stem cell potential.
For determining the specificity of the ELISA, ROC curves were generated as described below.
To determine the specificity of the PAI-1 mRNA response, we then determined uPA and PAI-2 mRNA abundances.
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