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For determining sequences of the plastid-encoded psbA/ rbcL spacer which lies upstream of the rbcL gene, i.e. between the psbA and rbcL genes, and full-length sequences of the rbcL gene the PCR approach of Andersen and Bailey [ 20] modified to amplify the target sequence in one piece [ 21] was used.
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GapCloser for SOAP de novo was used to determine sequences of the gaps in scaffolds [ 45].
For several of these apparently significant tags, we also determined sequences of the corresponding cDNAs and then confirmed the differences' reliability by real-time PCR.
Comparison of the independently determined sequences of the two BAC clones with the assembled genomic sequences showed perfect alignment with correct order and coverage, demonstrating that the coverage and quality of the assembled genomic sequences were high.
The phasors are used to determine the sequences of the signals.
We determined the sequences of the VH and VL regions of our anti-mTF monoclonal antibody.
As indicated in Table 1, three methods were used to determine the sequences of the 29 green algal chloroplast genomes.
A key goal of our future studies will be to validate this model by determining the sequence of the DNA bound to the AYVV CP, and thereby the number or position of these loops within the genomic DNA.
That is the price that a start-up company called Complete Genomics says it will start charging next year for determining the sequence of the genetic code that makes up the DNA in one set of human chromosomes.
Clinicians can identify increases in disease risk for specific patients, as well as their responsiveness to certain drugs, by determining the sequence of the billions of building blocks, called nucleotides, that make up their DNA.
By determining the sequence of the repeats, a profile is constructed which can be used for clustering.
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