The cytotoxic effect of DOX-loaded AQUA LNTs has also been determined on cell cultures.
In vivo effects of GPM1 were determined on cell surface presentation of gp96 and DC maturation associated with SLE.
Cell differentials were determined on cell smears stained with May-Grünwald-Giemsa stain.
Cellular CD26 or CXCR4 protein levels were determined on cell monolayers at 4 °C as previously described [ 32].
Alkaline phosphatase activity was determined on cell aliquots by substrate hydrolysis with p-nitrophenyl phosphate, and osteocalcin release was determined in cell supernatants with an enzyme immunoassay as described previously [ 23, 34].
Alkaline phosphatase activity was determined on cell aliquots by substrate hydrolysis using p-nitrophenylphosphate (PNPP), and osteocalcin release was determined in cell supernatants using an EIA as previously described [ 11, 12].
SY monolayers were used within the seventh passage in culture: u-PA and plasminogen activator inhibitor-1 (PAI-1) were assayed on supernatants, and urokinase-plasminogen activator receptor (u-PAR) was determined on cell lysates.
The protein was determined on cell lyses with 0.5 mL 0.1 M NaOH by the modified Lowry protein assay reagent (Pierce, Rockford, IL, USA) according to the manufacturer's manual.
VEGF protein induced pancreatic cancer was determined on cell-free supernatant by ELISA.
Cell autolysis rates were determined on cells exposed to linoleic acid using an assay modified from that described by Blackman et al. [82].
