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Due to the limited amount of liver tissue obtained from the biopsy sampling procedure additional assays, such as gel-shift assays providing direct evidence for activation of Nrf2 through determining of binding of Nrf2 to the ARE motif of Nrf2 target genes, could not be conducted.
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One likely explanation for these findings is that the determining feature of binding is an unknown substructure(s) of amylose and amylopectin, and starch "fingerprint -amylopectin chains, fingerprint -amylopectinpositioned branchainsnts.
The use of the structurally unrelated TG2 inhibitor ERW1041E served as a means of determining selectivity of binding of TG2 radiotracers in tumour tissue.
In this context, the ability of SPR technique to determine kinetics of binding is crucial.
Wild-type and mutant Vps1 were expressed, purified, and incubated with yeast F-actin and centrifuged to determine extent of binding.
Fluorescence and absorbance spectroscopies provide a powerful means of determining drug DNA binding constants.
We also aimed at determining whether binding of hFasL to the Fas receptor is required for hFasL cleavage.
The solvation energy of M+-DB18C6 in aqueous solution plays a key role in determining the binding selectivity of DB18C6.
The selection strategy is very important in determining the binding characteristics of the phage [ 21].
Overall, the EMSA assays suggest that the binding motif is the most important factor determining the binding activity of promoters.
First, the minor or major conformation of CFP determines the binding of ATP to the histidine.
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