Sentence examples for determining integration from inspiring English sources

This review focuses on the method for preparing infectious virions containing the IN fusion proteins and on the quantitative PCR assays for determining integration site specificity.

The resulting PCR products can be directly sequenced to determine integration sequences.

To determine integration of the vgb gene into the T. reesei genome, PCR was performed using the genomic DNA isolated from TU-6 and the selected transformants.

10.7554/eLife.05357.014 Figure 7. Polar localization determines integration of the nitrate reductase complex in anaerobic respiratory chains.

To determine integration of the molecular switch sequence, a genomic extraction of NIH3T3 cells was collected and analyzed by nesting PCR.

DOI: http://dx.doi.org/10.7554/eLife.10565.015 To infer the mechanism of genomic integration of transposon reporters, we analyzed the sequences of the insertion loci to determine integration preferences at base-pair resolution and identify potential sequence preferences.

The assay for determining XMRV integration sites in DU145 cells was performed as described previously [14].

In summary, we established a simple and reliable genotyping PCR method for determining the integration site of the MYCN transgene.

Therefore, the key to network analyses is determining which integration strategy should be implemented.

Once the cluster membership of the optimal integration for a data set was determined, the integration and determination of CA from the selected clusters were repeated 100 times with five-fold cross-validations.

To detect EBV integration, portions of the aligned molecules extending beyond the EBV map were extracted and aligned with hg19 to determine potential integration sites (Additional file 1: Figure S9).