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The Delphi method is largely used in the healthcare setting as a reliable means of determining consensus for a defined clinical problem [28 30].
We feel, however, that weighing individual amino acids in their contribution to overall peptide array phosphorylation seems a valid tool for determining consensus sequences.
It is generally accepted as an objective and rigorous method of determining consensus.
However, no standard method or appropriate guidelines are available for determining consensus levels [ 57- 60].
29 To address this, we sought statistical advice and defined specific criteria for determining consensus thresholds a priori.
However, since there was no aim of determining consensus, this is unlikely to have affected the outcome of the study.
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I think that if the two remaining contenders are John McCain (fiercely independent) and Barack Obama (determined consensus builder), the country might be in store for a refreshing change in conversation as to how do we fix our problems together from how do we create more problems through alienation.
In an effort to determine which assessment best represents the experimental data, weighted means statistics are used to determine consensus estimators for the data.
Although we were able to align and determine consensus primer sets for the 9 tri5 and the 16 β-tubulin sequences, there was no consensus sequence that could be derived from alignment of the 11 rRNA sequences.
Ten to twelve clones were sequenced per PCR product to determine consensus sequences [16].
By Sanger sequencing, we determined consensus whole-genome sequences for A/H1N1pdm viruses sampled nationwide in Canada over 33 weeks during the 2009 first and second pandemic waves.
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