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The supporting information on plasmid evaluations in Method S2 describes the use of cloned virus sequences for the preliminary selection of mutation-specific primers and determining assay absolute detection limits.
The original TaqMan Protein Assay is designed to quantify protein expression, making a purified protein target the most suitable control for determining assay performance.
This rapid pace makes upgrades, whether in software or hardware, difficult to avoid in large studies making it important to utilize randomization and blocking when determining assay processing order.
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Characteristics of the patients (n = 11) whose sera were used to determine assay specificity is shown in Table 1.
Lastly, a test sample collection was assayed to determine assay accuracy and precision.
As a control to determine assay background, assay buffer was added instead of microsomes.
and 4 known negative control serum samples were used to determine assay cutoff.
Moxifloxacin (Avelox®; Bayer) [21] was included as the positive control to determine assay sensitivity [22].
An HIV-positive with the Determine assay was confirmed positive on the STAT-PAK assay.
Plasma that tested positive by Capillus assay was confirmed by a Determine assay and vice versa.
An HIV-negative result with the Determine assay was reported as negative.
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