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The results suggest that the frequency of the amino acid residues at each position of the peptide enriched from the selection reflects that position's importance in determining affinity to the bait.
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To find out whether the cre box position in relation to the promoter plays a role in determining the affinity to CcpA, the distance between cre boxes and the corresponding transcription start sites (TSS) was analyzed.
The parameters and factors determining affinity partition have been evaluated.
Thus, surface plasmon resonance analysis utilizing BIAcore was performed to determine the affinities to the conserved phosphopeptides.
The first ten BBB-ChT-active bis-pyridinium cyclophanes were determined by their binding affinity to the BBB-ChT in a rat brain and were assessed according to their inhibition of [H] choline uptake.
To determine K 1, we measured binding affinity to the half-site REs in triplicate and fit the data to a single-site binding model.
Using an in-house, fluorescence-activated cell sorting (FACS) -based MHC stabilization assay [36], we determined the binding affinities to the MHC class I allele HLA-Cw*0102 HLA-Cw*0102 43 nofameric peptideset
"If you find a palm fossil and can determine its affinity to a modern subgroup of the palm family, you can, using our data, determine the temperature of the climate of when that palm was growing," explained Reichgelt.
Instead, the amount of bound bisphosphonate, determined by the affinity to bone mineral of the bisphosphonate used, the concentration of the bisphosphonate solution and the soaking time, seems to be more important.
Substrate-dependent ATP hydrolysis by a partially purified ubiquitin conjugation enzyme system from reticulocyte extracts was used to determine the enzyme affinity to calmodulin.
Note that relative, rather than absolute, affinity was determined due to the difficulty in determining the active fraction of the purified GST-PTB.
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