Sentence examples for determining affinity of from inspiring English sources

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Streptavidin interacts with its cognate molecule (biotin/desthiobiotin) and any unreacted, free streptavidin from first step will react with biotinylated BCCP determining affinity of streptavidin for biotin/desthiobiotin. Figure 6b shows that streptavidin interacts efficiently with both biotin and desthiobiotin.

The duplex forked structure used in determining affinity of XPD for the forked structure was annealed as described in Pugh et al (2008a).

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By determining affinities of these ligands for various regulatory state SFK mutants, one can determine the influence of intramolecular interactions on ATP-binding site conformation.

In a painstaking series of some 10,000 experiments, he determined affinity of 35 different binding sites within the MHC clefts for the 20 naturally occurring amino acids that make up peptides.

We next used these measurements to quantitatively analyze the genetic factors within the RE's DNA sequence that determine affinity of AncSR1 and of AncSR1+RH.

To begin to investigate how modifications impact allostery in KMD5A, we performed competition-based FP assays to determine affinities of a panel of modified peptides (Fig. 4a).

Therefore, the determined affinity series of metal ions for lignin is as follows: Pb II) > Cd II) > Cu II).

CGP 12177 has been labelled with radioisotopes (Staehelin et al., 1983; Dubois et al., 1996) and used extensively to determine affinity values of unlabelled antagonists at the high affinity catecholamine site (Joseph et al., 2004; Baker, 2005).

Hunter, S. A. & Cochran, J. R. Cell-binding assays for determining the affinity of protein protein interactions: technologies and considerations.

Indeed, the control of ligand orientation on the nanoparticle surface is of crucial importance in determining the affinity of the immobilized biomolecules toward the biological target.

The role of apoptosis in affinity maturation was investigated by determining the affinity of (4-hydroxy-3-nitrophenyl)acetyl (NP -specific aNP -specificing cells (AFCs) and serum antibody in transgenicellse thAFCsverexpress anduppreserumof antibodys, Bcl-xL, in transgenic comicethatt.

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