"Most people thought this was too far out in the future, to actually determine a structure," Dr. MacKinnon said.
"Strain is a very important parameter," he says, and often determines a structure's stability and why it grows the way it does.
The model obtained was used to determine a structure solution for the 2.8-Å data.
We obtained crystals of phosphoUb and determined a structure to 1.9 Å resolution (Table 1).
It is quite rare these days to determine a structure of a soluble protein and really find something new.
To determine a structure with the intact donor substrate bound, the predicted nucleophilic residue, Asp394, was mutated to Ala.
(162) As in protein crystallography, it is usually much quicker and easier to determine a structure if a sufficiently similar starting model is already available.
The TtrD protein was overproduced and purified, and attempts were made to determine a structure of the complex with a TtrA signal peptide.
The 2D NOESY spectra yielded few small NOEs with the same sign as the diagonal (spin diffusion regime) that were insufficient to determine a structure consistent with that reported previously for polymyxin B in water.
Research about specific characteristics of health plans and effects on health outcomes is needed to determine a structure of insurance coverage that provides optimal access to specialty care for children.
Only with additional research about the specific characteristics of health plans and the effects on health outcomes will it be possible to determine a structure of insurance coverage that provides optimal access to specialty care for children.
