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One limitation of the study is the small number of patients in each group which does not allow for determining a significant difference in biofilm production by H. influenzae between strains from healthy controls and the MEF group (children with AOM treatment failure).
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Applying the radiographic score system we determined a significant difference between experimental groups and controls without cells.
It has been calculated that 27 early end-points will be sufficient to determine a significant difference between the groups.
Assuming an effect size of 0.7 from previous reports, a total sample size of 40 animals was calculated to determine a significant difference between SEP amplitudes with an alpha error of 0.05 and a power of 0.95 between the four groups.
Annual mean POC concentrations did not significantly differ across the WCAs (Fig. 4; χ 2 = 4.36, df = 2, ρ = 0.11); however, the multiple comparison test determined a significant difference of POC concentrationbetween WCA-1 and WCA-2 (z score = − 2.06, ρ < 0.05).
To determine a significant difference between the recovery curves, the area under a curve (AUC) was analyzed by first restructuring data in SPSS 14.0 followed by computing the AUC in NCSS 2007.
Data were analysed using Student's t-test to determine a significant difference between sample means.
With these results we could not determine a significant difference between the ICG dose nor the administration site.
Following the experimental protocol, we performed systemic hemodynamics, pulmonary hemodynamics and other variables to evaluate whether we could determine a significant difference between groups.
The increase in MPAP and PCWP was considered high during surgery, but in observations during postsurgery we could not determine a significant difference [ 16].
Regarding the number of cases, we were unable to determine a significant difference between both datasets, the difference in the Pearson chi-squared statistics of our log-linear models being not significant (p = 0.19).
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