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We then used the miRHub algorithm (Methods), which determines whether the predicted regulatory effect of any given miRNA on a specific set of genes is significantly greater than expected by chance (i.e., acts as a "regulatory hub"), to predict whether any of these miRNAs are candidate drivers of the gene expression profiles associated with the phenotypes of interest.
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Expression vectors for these proteins were introduced transiently into HCT116 cells and subjected to western blotting with anti-HA antibodies to determine whether the predicted recombinant CHFR proteins were produced (Fig. 1B).
PCS becomes, therefore, a criterion for determining whether the predicted transcript should be recorded.
34 The protein variability index was used to determine whether the predicted epitopes were positioned in the least variable, moderately variable, or hypervariable regions.
Sequences retrieved were subjected to a TargetP analysis to determine whether the predicted proteins were likely to be localised to mitochondria or microsomes.
42 To determine whether the predicted unintended consequences of plain packaging could be detected between 2011 and 2013, we used logistic regression.
The purpose of this regression analysis was to determine whether the predicted and observed values obeyed the equation yi= yi* (i.e., whether the points fall on the line of equality).
The in silico validation made use of the available A and D diploid genome sequences by determining whether the predicted aa/bb dual homozygous markers had the expected evolutionary pattern for sequences from Scenario 1 (Fig. 1).
The putative microsomal CBR sequences were then subjected to a TargetP analysis (Emanuelsson et al., 2000) (accessed via: http://www.cbs.dtu.dk/services/TargetP/) to determine whether the predicted proteins were potentially localised to mitochondria or the endoplasmic reticulum.
Third, the output structures from I-TASSER are then submitted to RosettaDock (Lyskov and Gray 2008) to determine whether the predicted monomeric MVP structures are expected to assemble into vaults.
In an attempt to determine whether the predicted Zur-binding site of the CC2720-26 promoter was functional and if Zur regulates this operon directly, site-directed mutagenesis was performed.
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