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The ControlType attribute determines whether the interaction represents activation or inhibition.
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To determine whether the interaction between OsSND2 and OsMYB61 promoter occurs through binding to SNBE site, we performed sequence searching within the promoter of OsMYB61 and found it contains two SNBE sites (SNBE1 and SNBE2) (Additional file 2: Figure S2a).
Initially, we wanted to determine whether the interaction between ApoEr2 and X11α affected cell surface levels of ApoEr2.
To determine whether the interaction with Rho GDI might regulate the levels of RhoA in the nucleus, we used siRNA to knockdown Rho GDI1 expression.
To determine whether the interaction between Tbx1 and Smad1 has functional consequences, we overexpressed Tbx1 and assessed the transactivation ability of Smad1.
Once it was determined that AKIP 1A, PKAc, and p65 interact in resting or serum starved cells, we next wanted to determine whether the interaction was formed in the cytosol or nucleus.
To determine whether the interaction of Toca-1 with N-WASP was important for the induction of membrane tubules and vesicles the W518K mutant of Toca-1 was used.
To determine whether the interaction between frataxin and the core complex is an essential function of frataxin in vivo, we generated four murine fibroblast cell lines stably expressing full length hFXNN146K, hFXNN146A, hFXNW155R or hFXNW155A in the conditional allele background enabling the deletion of the endogenous gene [30].
We used MDA to separate the different structures, used the number of class members (raw 3-D repeats) to quantify the numbers of cross-bridges of a particular type and used quasiatomic model building to determine whether the interaction was strong or weak (this process is defined below).
It is also important to determine whether the interaction can be inhibited specifically.
Determining whether the interaction is indeed legitimate is a complicated question.
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