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This technique determines the expression of a target gene by comparison to an internal control gene uniformly expressed among the samples analyzed.
Rearrangement of the IgH locus determines the expression of the pre-B cell receptor (pre-BCR) and the transition of pro-B cells to the pre-B cell (B220loCD2+) stage [2].
To validate our methodology for inferring regulatory phrases we use the nature of the regulation already reported in the data bases for the case of simple regulations, namely, when only one regulator determines the expression of the regulated gene (gene A is only regulated by gene B).
In as much as elevation in intracellular calcium levels determines the expression of NMDAR-dependent hippocampal LTP and LTD [15], both of which are protein synthesis dependent [16] [17], changes in cytosolic calcium concentration may be intrinsically involved in the cellular mechanisms underlying information storage in the mammalian brain.
FucT I (H enzyme) preferentially fucosylates type 1, whereas FucT II (Se enzyme) determines the expression of H-type 2 (Fucα1-2Galβ1-4GlcNAc Fucα1-2Galβ1-4GlcNAc Fucα1-2Galβ1-4GlcNAc
First of all, through regulation of decisive axonal surface proteins namely NRG1 type III the Nf2 gene product determines the expression of growth factor-like molecules on axons important for Schwann cell actions such as proliferation and myelination.
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Western blotting was carried out to determine the expression of LC3-I and LC3-II in HEK293T cells ectopically expressing various coronaviral PLPs (Fig. 3A).
We also determined the expression of cadherins relative to EMT.
These tissue lysates or RNA were used to determine the expression of Nrf2, Cldn4 and AhR.
ELISA and RT-PCR determined the expression of IL-l2.
As well, we sought to determine the factors that determine the expression of lactate transport proteins and their cellular domains.
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