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Transcriptional control through isoform selection and mRNA levels determines pathway activation and ultimately susceptibility to disease.
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First, genetic studies of sex determination in laboratory mice have shown that the sex determining pathway is sensitive to genetic background [ 48- 51].
Using a novel gene clustering strategy we determined pathway relationships from a genome-wide transcriptional dataset of skeletal myoblast differentiation.
With the determined pathway, we propose that the hydrogenation of benzene on Ru(0 0 0 1) follows the mechanism with the step-wise hydrogenation of neighboring C atoms in the ring, i.e., 1 2 3… hydrogenation.
Pathway Express calculates a probability (P) value, based on the density of genes that belong to a determined pathway using the Kyoto Encyclopedia of Gene and Genome database [55].
Nuclear localisation of CTNNB1 was used to determine pathway activation.
We applied two methods to calculate gene score association, and three analyses to determine pathway enrichment.
Expression of Sry activates Sox9 and the male determining pathway leading to Dmrt1 (Koopman 2005).
Gene Set Enrichment analysis uses a priori defined gene sets to determine pathway differences between two phenotypic states.
After determining which reactions were functional, the reaction list was perused to determine pathway and subsystem functionality in the network.
However, current methods rely on statistical tests to determine pathway relations, and may lose numerous biologically significant relations.
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