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To further consolidate this hypothesis, we performed an established test to measure depression in rodents, the tail suspension test, which determines over 6 min the time mice spend immobile.
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Gene Trail is an advanced gene set enrichment analysis tool that determines over-represented gene ontology categories in data sets [13].
Gene Set Enrichment Analysis (GSEA, [ 41]) was used to determine over-represented genes and their GO categories.
The binomial test was used to statistically determine over-representation of PANTHER classification categories [ 33].
A cutoff of 2-fold was used to determine over-expression.
A GOslim analysis was performed to determine over-representation of molecular function in selected groups.
GSEA was used to determine over- or underrepresented genes and their GO categories.
PANTHER Classification System was used to statistically determine over- or under- representation of pathways http://www.pantherdb.org/[ 18].
The MIPS Functional Catalogue Database (FunCatDB) [ 54], was used for determining over-represented cellular functions in each gene list containing a particular word.
The hypergeometric distribution was employed to determine over-represented categories with the network analysis tool compare-classes (http://www.rsat.eu/).
His worth will be determined over time.
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