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The geNorm 3.5 visual basic application (VBA) applet for Microsoft Excel was used to determine the most stable housekeeping genes (http://medgen.ugent.be/~jvdesomp/genorm/) as previously developed and validated by Vandesompele et al. [ 80]. geNorm determines an expression stability score (M) of a housekeeping gene as the average pairwise variation V for that gene from all the others.
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CEO of Professional Science Editing for Scientists @ prosciediting.com