At the indicated time points (3, 24, 48, 72 h) after infection, supernatants were removed, cells were lysed and serial dilutions of lysates were plated to determine CFU, which represent intracellular bacteria.
Fortunately, we observed both compounds in most of the pool water concentrates, so it just remained to be determined which specific isomer represented each GC/MS chromatographic peak.
Comparing the linear coefficient (RLin) and logarithmic coefficient (RLn), for each child, it could be determined which model best represented each child's mental representation.
Together, the sheer number of changes in the expression of microenvironment constituents that associate with the senescence gene expression programme provides challenges in determining which alteration represent the dominant influences on adjacent cell types.
Future work using RACE-PCR from primers based on the transcripts identified here would produce full-length cDNA sequences that will determine which transcripts represent unique genes and which are fragments, Such genes could then be expressed into insect cell cultures for use in downstream enzymatic activity assays [ 85].
The putative SppuDAB sequences form one clade that includes the DAB sequences previously isolated from cDNA (Miller et al. 2005), but relationships within the clade are not well-resolved, and it is not possible to determine which sequences represent orthologous loci.
We also determined which mouse samples were represented in each of these groups.
Then, using additional data from the IMF in combination with Flurry's own data, the firm was able to then determine which countries represented the top market opportunities for mobile app developers.
The proposed model, analogously to previous approaches [26], uses exception resolution trees to determine which exception represents a set of exceptions raised concurrently in a certain context.
To determine which clades represented phylogenetic species, we applied the Genealogical Concordance Phylogenetic Species Recognition concept according to which well supported, terminal clades in multigene phylogenies are recognized as phylogenetic species [38].
