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We next determined whether the identified coexpression chromosomal domains correlated with any known cytogenetic bands on the chromosome.
Because adipose tissues are composed of several cell types, we determined whether the identified genes were expressed in mature adipocytes or nonadipocyte cells that constitute the SVF.
Firstly, the co-expression network analysis identifies associations rather than causal links between molecular changes and disease parameters, so it cannot be determined whether the identified modules are directly driven by intervention or an indirect association.
In addition, we determined whether the identified miRNAs (miR-16, miR-25, miR-92a, miR-451 and miR-486-5p miR-486-5p miR-486-5pNactasd are excreted into the culture media by BGC823 and MGC803 cell linesecretory
The median expression levels of the single proteins in the two tumour groups are listed in Table 3. Next, we determined whether the identified HSP/GRP protein patterns correlated with CTX responses, particularly with respect to histopathological tumour regression and tumour down-categorisation.
The fact that indirect interactions are readily detected by ChIP complicates its use in motif definition, as it cannot be determined whether the identified motif is that of the protein being affinity purified, an associated protein, or a composite of factors.
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The team is now planning follow-up studies with radio telescopes to determine whether the identified sources are emitting their light as a series of brief light pulses – the trademark that gives pulsars their name.
Each input data is randomized to determine whether the identified subsequences are significant or not.
A co-immunoprecipitation (co-IP) assay was employed to validate the yeast two-hybrid approach and to determine whether the identified interactions occur in human cells.
To determine whether the identified putative forkhead binding element in PII was bound by FOXL2 we performed an EMSA using 38bp probes centered on the site (Fig. 5A).
Our purpose was to determine whether the identified DEGs were able to serve as a gene signature to classify patients into their corresponding cancer type groups.
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