We then deleted each reaction and determined viability on each of the 10 carbon sources.
To determine viability of H295R cells after PLK-1 transfection, H295R were transfected with PLK-1, negative-control or UBBs1 siRNA and assayed for viability after 72 h.
Briefly, cells were seeded 2.5 × 104 cells/well at temperature maintained 37 °C and allowed to grow undistributed for 24 h then cell counts were made by using the Trypan Blue exclusion method to determine viability of cells [18].
Following completion of the incubation period, the eggs were candled to determine viability of the embryo and then placed at 2 8°C for a minimum of 8 hours.
This assay is based on the reduction of resazurin to resorufin as an indicator for metabolic activity which has been used previously to determine viability of T. denticola [22], [23].
Studies are needed to determine viability of T. cruzi in açaí, along with the tree-to-bowl continuum of açaí, to identify sources of contamination.
In this sense, species-energy theory [ 27] predicts that available energy determines viability of rare species and consequently community richness [ 14, 28].
Specifically, we chose a metabolic reaction at random and equiprobably among all reactions, deleted it, and used FBA to determine viability of the resulting metabolism.
In order to determine viability of mPCKS, ATP levels per mg of protein were assessed directly after preparation of slices, and after 1, 6, 12, 24, 48 and 72 h of incubation (Fig. 1A).
The use of vital staining to determine viability of T. vaginalis in studies involving interaction with antimicrobial peptides is not a suitable methodology, since these molecules alter the cell membrane making it more permeable to the colorant [ 4].
