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Vaccine shelf life is best determined utilizing a formal statistical evaluation outlined in the ICH guidelines, while minimum release is calculated to help assure adequate potency through handling and storage of the vaccine.

Sugar and moisture values were determined utilizing a special refractometer Bertuzzi (Bertuzzi, Milano, Italy) owing two direct reading displays, for the measurement of sugar content and moisture percent respectively (Chatway method).

Therefore, in a swine model, the efficacy of CO2 removal at a dialysis flow rate using regional trisodium citrate anticoagulant and also systemic anticoagulant was determined utilizing a modified renal dialysis unit.

Sugar and moisture values were determined utilizing a Bertuzzi refractometer (Bertuzzi, Milano, Italy) owing two direct reading displays, for the measurement of sugar content and moisture percent respectively (Chatway method).

On day 7 the medium was replenished and on day 14 the p24 levels were determined utilizing a standard p24 ELISA assay, with the TCID50/ml calculated.

The relative quantity of NiV RNA was determined utilizing a Taqman probe that targets the NiV nucleoprotein (N) gene and detects cDNA derived from both genomic and positive sense RNA [23].

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Because the ultimate objective in utilizing active composites centers on their ability to deform, optimal parameter values were identified employing analytical fiber and lamina strain/electric field models and limitations were determined utilizing an analytical embedment stress model.

RNA quality and integrity were determined utilizing an Agilent 2100 Bioanalyzer (Agilent Technologies) and a NanoDrop spectrophotometer.

Finally PFI was determined utilizing an adjusted nomogram" where aerobic fitness and muscle fitness are equally important (50 and 50%).

RNA quality and integrity were determined utilizing an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) and absorbance at A260/A280.

Grb2 localization was determined utilizing an imaging approach with acquisition of 12 images/well and scoring for Grb2 positive pit formation.

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