The optimum operating conditions have been determined using the step adjusting gradient method.
Furthermore, the histogram of pause durations determined using the step-finding algorithm showed a nonexponential distribution with an initial lag phase, and the average pause duration increased with the increase in GC content.
We then built transition density plots (McKinney et al., 2006) that represented the two-dimensional histogram for pairs of FRET values, determined using the step-finding algorithm, before and after each pause.
The level of fold change and statistical significance (≥ 3-fold change, P < 0.05) were determined using the step up Benjamini-Hochberg post-hoc test for multiple corrections following one way ANOVA.
There is no significant difference between the magnitudes of the shared KIE2 (ca. 4) determined using the 2-step and 3-step models and the value of this KIE is in good agreement with that obtained by fitting the [D4]EAL versus [D2]EA transients to a double-exponential function (Table 2).
In the entropy measurement approach, the weights of criteria were determined using the following steps (Aomar, 2002): Formulation of normalized pay-off matrix (P ij ) : The criteria matrix (Table 1) was normalized using the equation (6).
The sets S [ 1 ] Open image in new window to S [ Z ] Open image in new window and functions F [ 1 ] t Open image in new windowto F [ Z ] t Open image in new window are determined using the following steps.
In the entropy measurement approach, the weights of criteria were determined using the following steps (Aomar, 2002): Table 3 Relative weights of criteria Cost Delivery Capacity Warranty Weights of criteria from literature (Cheraghi, 2004) 0.2337 0.2896 0.2352 0.2415 Weights of criteria by using entropy measurement 0.2445 0.2445 0.2445 0.2445.
To quantify the stepwise patterns observed in single molecule FRET-time traces for G4 unwinding, average EFRET values and the dwell time of each unwinding step were determined using the automated step-finding method used in previous single molecule FRET studies (Kerssemakers et al., 2006; Myong et al., 2007; Lee et al., 2012).
Our aim was to study the association between self-reported PA (Short QUestionnaire to ASses Health enhancing PA) and fitness (determined using the Siconolfi step test) among sedentary women in a multiethnic population.
