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The phosphate concentration was determined using the recommended protocol supplied with the PiColorLock gold phosphate detection system from Innova Biosciences (Cambridge, United Kingdom).
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The date of death was determined using the procedure recommended by the GPRD.
The titer and percentage of recombinant phages in the library were determined using the protocols recommended by the manufacturer.
Minimal inhibitory concentrations (MIC) of vancomycin were determined using the general protocol recommended by the Clinical and Laboratory Standards Institute (CLSI, 2008).
The required minimum sample size for the study was determined using the following formula recommended by the WHO for cluster surveys of immunization coverage [ 15]. n = D E x z 2 x p x 1 − p d 2 In the calculation of the sample size, the Design Effect (DE) was taken as 2. The Standard Normal Deviation (Z) corresponding to the 95% confidence interval was 1.96.
These isolates were identified using standard microbiological techniques and their antibiotic susceptibility was determined using the disk diffusion method recommended by the French Society of Microbiology.
The ring growth velocity, which is a median value of its reasonable range determined using the advanced feasible forming condition, is recommended for the forming process design considering the reduced ring spreads and uniform plastic deformation.
Protein concentration was determined using the Bio-Rad protein reagent, as recommended by the manufacturer, with a BSA standard curve.
IL-8 was determined using the duoset capture and biotinylated detecting antibody as recommended by the manufacturer (Invitrogen).
Plasma fructosamine levels were determined using the Kamiya Biosciences (Seattle, WA) Fructosamine Assay Kit, following the manufacturer's recommended protocol.
T is automatically determined using the CMIF.
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