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Protein concentrations were determined using the method of Bradford (Biorad, Veenendaal, the Netherlands).
To this end, pain thresholds were determined using the method of limits.
The differential expression of genes at different stages of brood pouch development was determined using the method developed previously45.
Chlorophyll contents were determined using the method of Poora (2002).
Total nitrogen was determined using the method of Jackson (1962).
Protein concentrations were determined using the method of Brandts and Kaplan [63].
The serum composition of fatty acids was determined using the method by Lepage and Roy [24].
First, the pain threshold was determined using the method of limits [21].
Protein concentrations of each sample were determined using the method described by Lowry et al [49].
It was determined using the method of Murphy, et al. [19].
Protein concentrations were determined using the method of Bradford (1976).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com